Imaging platform for cell and tissue analysis.
Research at Institut Armand-Frappier is aimed at determining the endocrine disruptor effects on germ cell development and mammary gland development. To this end, we use rodent, cell culture and 3D culture models. We also have the necessary equipment to analyse tissular and cellular effects, by imaging and analysis of fluorescent biomarker expression.
Imaging, cell and tissue analysis: tissue preparation for histology, cryosections, stereomicroscopy, light microscopy, fluorescence microscopy, confocal and spectral microscopy, cell sorting, fluorescence analysis, analysis and imaging of living cells.
The infrastructure is used for the following purposes:
- Histology (paraffin coating, microtomes and cryostat)
- Microscopy (stereomicroscopy, optical microscopy, fluorescence microscopy and confocal microscopy)
- Flow cytometry cell sorting (six colours, eight parameters)
- Automated image analysis combined with a multimode plate reader
- Real-time cell population analysis
- Agriculture, animal science and food
- Chemical industries
- Education
- Environmental technologies and related services
- Healthcare and social services
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
|
Equipment |
Function |
|---|---|
|
Paraffin coating platform |
Tissue coating |
|
Microtome |
Histological sections |
|
Cryostat |
Cryosections |
|
BD Biosciences FACSJazz cell sorter |
Flow cytometry cell sorter (six colours, eight parameters); sorting of two populations or on culture plates |
|
BioTek Cytation 5 |
Automated image analysis system combined with a multimode plate reader |
|
Nikon confocal A1R+ microscope |
Confocal microscope with a spectral detector and incubation chamber for analysing live cells |
|
Leica LED3000 stereomicroscope |
Stereomicroscope equipped with a camera, available under aseptic conditions |
|
Zeiss Primostar optical microscope |
|
|
Nikon Eclipse Ti-E inverted fluorescence microscope |
|
|
xCELLigence RTCA systems |
Real-time analysis of cell proliferation, death and migration |
Additional information
|
Title |
URL |
|---|---|
|
Cytation 5 demo video |
|
|
Information about xCELLigence |
|
|
Prof. Isabelle Plante |
|
|
Prof. Géraldine Delbès |