Molscreen (ligand discovery and characterization)

University of Alberta, Edmonton, Alberta
What the facility does

Research that relies on biophysical instrumentation for discovery and cross-validation of lipid ligands and small molecule drug leads for proteins, memteins and native membranes.

Areas of expertise

Molscreen offers access to a suite of biophysical instruments, training and analysis to screen and cross validate interactions of lipids, proteins and drug molecules. This allows the measurement of their binding affinities, stoichiometries and ligand specificities for water-soluble and membrane-associated proteins and macromolecular assemblies using native nanodisc and styrene maleic acid lipid particle (SMALP) technology.

Research services
  • Access to biophysical instrumentation to characterize biomolecular interactions
  • Training on the use of biophysical instrumentation to measure interactions between proteins, membranes, lipids and drug molecules
  • Collaborative research on targets including oncogenic proteins and receptors
Sectors of application
  • Life sciences, pharmaceuticals and medical equipment
GatorBio GatorPlus Bio-Layer Interferometry (BLI) systemWith 8 channels to sample 96 and 384 well plates with minimum volumes of 40 microlitres. Label-free, real-time measurement of protein interactions with nanodiscs, liposomes, antibodies, drug molecules, binding constants, ligand binding specificities, lead determination.
Malvern Panalytical MicroCal PEAQ-ITC isothermal titration calorimeterWith sample volume of 280 microlitres. Direct, label-free, in solution measurement of proteins and ligands, determination of binding constants, stoichiometry, enthalpy and entropy of biomolecular interactions.
Malvern Panalytical Zetasizer Ultra Zeta Potential AnalyzerMeasurement of nanodisc sizes, charges, concentrations and multiangle dynamic light scattering.
Fluidic Analytics Fluidity One-M systemReal-time measurement of affinity, size, stoichiometry of molecular interactions of nanodiscs and proteins, both purified and in cell lysates.
Pavonis Diagnostics Visual Interference Color Assay (VICA) Protein Detection SystemLabel-free measurement of binding affinities and kinetics of interactions of proteins.
  • Cube Biotech
  • Molsoft
  • Pavonis Diagnostics
Discovery of allosteric SHP2 inhibitors through ensemble-based consensus molecular docking, endpoint and absolute binding free energy calculations.
A conserved epitope in VAR2CSA is targeted by a cross-reactive antibody originating from Plasmodium vivax Duffy binding protein.
Effects of specific inhibitors for CaMK1D on a primary neuron model for Alzheimer's disease.
How choice of model membrane affects protein-glycosphingolipid interactions: insights from native mass spectrometry.
Effects of specific inhibitors for CaMK1D on a primary neuron model for Alzheimer's disease.
Structural basis for recognition of transcriptional terminator structures by ProQ/FinO domain RNA chaperones.
Membranes are functionalized by a proteolipid code.
Recognition and remodeling of endosomal zones by sorting nexins.