Cellular biology research of diseases related to the diet and the digestive tract
The Diet, Digestive tract, & Disease (3D) CFI-ORF Basic Science Research Facility is a centre of excellence in fundamental biology and health research. We specialize in cell-based assays, biochemical assays, cellular and small animal in-vivo time lapse and 3D imaging in full level 2 biosafety containment. The CFI 3D facility instrumentation consists of state-of-the-art equipment providing cutting-edge data acquisition and analysis in a user-friendly environment. We provide services on these core instruments consisting of usage, training, full assistance and assay development, at a very competitive cost.
The (3D) CFI-ORF Basic Science Research Facility is also associated with the Diet, Digestive tract, & Disease (3D) CFI-ORF Clinical Research Facilities. Both are part of the larger multidisciplinary research infrastructure of the 3D initiative that spans the University of Toronto, St. Michael’s Hospital, and the Sunnybrook Health Sciences Centre.
FRET-based assays, automated calcium imaging, luminescence and absorbance assays, high capacity and high throughput microscopy and screening, X-ray (bone density, peripheral fat measurement) bioluminescence and fluorescence, non-invasive in vivo imaging, multiplex measurements, hormones, cytokines and chemokines measurements
- Agriculture, animal science and food
- Fisheries and aquaculture
- Life sciences, pharmaceuticals and medical equipment
- Manufacturing and processing
Specialized labs and equipment
| Name of specialized lab | Name of equipment | Description of function |
|---|---|---|
| The 3D CFI facility | BMG PHERAstar FSX | Versatile and FRET-compliant plate reader |
| Thermo Scientific Cellomics ArrayScan VTI | High-capacity and fully automated single cell fluorescent microscopy platform | |
| PerkinElmer IVIS Spectrum | Bioluminescent and fluorescent in vivo 3D imager in Biosafety Level 2 approved animal room | |
| Meso Scale Discovery SECTOR Imager r 2400A | Multiplex reader | |
| Bruker In-Vivo Xtreme | Radioluminescent, bioluminescent and fluorescent in vivo 2D imager in an approved animal room (digital high resolution X-ray, bone density & peripheral fat measurements) | |
| Nikon A1R multi-photon microscope | Ultrafast Multi-color 2-photon microscope in an approved animal room | |
Nikon Swept Field confocal microscope | Live cell imaging with a High sensitivity and High Speed detection fluorescence microscope | |
Applied Biosystems ViiA 7 rtPCR system | Highly productive and user friendly system | |
| Beckman Coulter Optima XPN-80 Ultracentrifuge | Ultracentrifugation up to 80000rpm or 548000rcf. Fully digital control console, with touch -enabled screen for easy programming | |
| Beckman Allegra X-15R centrifuge | Allegra X-15R—Benchtop centrifuge. |
Additional information
| Title | URL |
|---|---|
| CFI 3D Facility web link | https://drive.google.com/file/d/1jCjF2VfYf6RdCJFsLDt6r-caY8sfhaPJ/view?usp=sharing |
| DCM web link | https://www.dcm.utoronto.ca/imaging |