Research to determine how modifications to starting materials affect quality attributes of nanomedicines and furthering the development of nanoscale reference materials
The laboratory’s work involves adapting existing methods and developing new ones to analyze and understand sub-visible protein aggregation, performing regulatory research to understand the mechanisms by which alterations to the individual components of a nanomedicine affect its overall attributes, performing research to identify limitations regarding methodology utilized to characterize nanomedicines and performing research to identify emerging concerns regarding the use of nanotechnology/nanomedicines and to subsequently provide scientific responses to those concerns in this rapidly advancing field. The laboratory is also actively involved in the development of novel methods for nanoparticle fabrication as well as the development of nanoscale reference materials.
The laboratory is capable of nanoparticle assessment via dynamic light scattering, single nanoparticle tracking analysis and resonant mass measurement. Additionally, the laboratory can also perform protein structure/stability assessment via circular dichroism spectropolarimeter, differential scanning calorimetry and isothermal titration calorimetry. The capability to characterize and quantify sub-visible particles in therapeutic formulations via micro-flow digital imaging analysis is also within the laboratory’s expertise.
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
| Equipment | Function |
|---|---|
| JASCO J-1500 Circular Dichroism Spectrophotometer | For assessing secondary and tertiary protein structure as well as protein stability |
| Malvern Panalytical NanoSight NS300 | Automated analysis of size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter |
|
Malvern Panalytical Archimedes Particle Metrology System |
Uses resonant mass measurement to detect and count particles between 50 and 5000nm |
| Fluid Imaging Technologies FlowCam VS series | A fluidics system that uses a flow cell and digital imaging system to characterize and count sub-visible particles |
| Thermal Analysis (TA) Instruments Nano ITC (Isothermal Titration Calorimeter) | A highly sensitive calorimeter capable assessing protein/protein or protein/ligand interaction |
Private and public sector research partners
International Pharmaceutical Regulators Forum (IPRF)
Additional information
| Title | URL |
|---|---|
| Biologics and Genetic Therapies Directorate | https://www.canada.ca/en/health-canada/corporate/about-health-canada/branches-agencies/health-produ… |
| Nanoparticle size and efficiency affected by presence of fatty acids during albumin nanoparticle fabrication. PLOS ONE |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189814
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| Toxicogenomics analysis of mouse lung responses following exposure to titanium dioxide nanomaterials. Mutagenesis |
https://academic.oup.com/mutage/article/32/1/59/3865758
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pHAST (pH-Driven Aptamer Switch for Thrombin) Catch and-Release of Target Protein. Bioconjugate Chemistry |
https://pubs.acs.org/doi/10.1021/acs.bioconjchem.6b00124
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| Evaluation of Microflow Particle Analysis for Sub-Visible Particles Formulated with Opaque Vaccine Adjuvant. PLOS ONE |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150229
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| Determination of Supplier-to-Supplier and Lot-to-Lot Variability in Glycation of +Re Hum Ser Alb Expressed Oryza sativa. PLOS ONE |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109893
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| Generation of fatty acids from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/cardiolipin liposomes. J Liposome Res |
https://www.tandfonline.com/doi/abs/10.3109/08982104.2012.747535?journalCode=ilpr20
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| Investigation of differences in thermal stability 2 recombinant human serum albumins with 1,2-dipalmitoyl-sn-glycero-3-Phos. J Phys Chem |
https://pubs.acs.org/doi/10.1021/jp2125729
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| O-linked glycosylation leads to decreased thermal stability of interferon alpha 2b measured by 2 orthogonal techniques. Pharm Res |
https://link.springer.com/article/10.1007%2Fs11095-011-0402-0
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| Influence of bovine serum albumin on secondary structure of interferon alpha 2b. Biologicals |
https://www.sciencedirect.com/science/article/pii/S1045105609001821?via%3Dihub
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| Characterization of retention and pharmacokinetic properties of liposomal nanoparticles dihydrosphingomyelin. BBA-Biomembranes |
https://www.sciencedirect.com/science/article/pii/S0005273607000314?via%3Dihub
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| Therapeutically rates of drug release achieved by varying drug to lipid ratio in liposomal vincristine. BBA-Biomembranes |
https://www.sciencedirect.com/science/article/pii/S0005273606000113?via%3Dihub
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