Bioprocessing and nanoformulations to produce high-value and safe bioproducts
The Bioprocessing and Nano-Enzyme Formulation Facility (BANEFF) works in close collaboration with universities and the industrial sector for the testing and commercialization of different technologies of bioproduction, such as enzymes, biohydrogen, butanol, citric acid and fumaric acid. In addition to the regular laboratory equipment for specific uses and with supportive analytical facilities, BANEFF comprises principally three phases: fermentation, extraction and formulation. The facilities are accessible to universities, industries and government laboratories. The three-phase installations comprise a liquid-state fermenter, two solid-state fermenters, lyophilizer, incubator shaker, ultrafiltration system and nanospray dryer, along with spectrophotometer, autoclave, nanosizer and allied equipment. The installations comprise a platform for upstream bioprocessing, downstream bioprocessing and peripheral analytical equipment. There are different ways in which clients can use the BANEFF infrastructure: (1) getting complete training on the equipment and using it themselves in the laboratory; (2) through research contracts with INRS-ETE; and (3) enhancing the research and testing potential by collaborating with the professor through different research-grant mechanisms or direct research contracts.
To date, BANEFF has carried out the diverse activities through direct training and industry-university research collaborations. BANEFF has also worked in partnership to produce enzymes for the brewery industry, biopesticides, value addition of pomace into diverse enzymes, antioxidants and animal feed, enzymes for lipid extraction and green-food alternatives.
Fermentation: solid and liquid state, pre-treatment of wastes, screening of strains for high-performance production of bioproducts, extraction and purification of bioproducts, microformulation and nanoformulation of bioproducts, analysis of unknown metabolites, degradation by-products of organic compounds in the environment for complex matrices such as wastes, waste water, sludge and soils
- Agriculture, animal science and food
- Energy
- Environmental technologies and related services
Specialized labs and equipment
Specialized lab | Equipment | Function |
---|---|---|
Bioprocessing Laboratory | Liquid-state fermenter, including hydrogen and CO2 sensor and mechanical foam breaker and chiller units | For fermentation of different substrates coupled with the measurement of gases during aerobic and anaerobic fermentation |
Bioprocessing Laboratory | Solid-state fermenter (rotating drum type), including in situ sterilization, measurement of moisture, pH and CO2 | For fermentation of solid and semi-solid substrates with total solids concentration exceeding 80 g/L |
Bioprocessing Laboratory | Autoclave for dry and liquid samples | Sterilization of dry and wet media for pure-strain fermentation and decontamination of petris/wastes |
Bioprocessing Laboratory | Portable benchtop laminar-flow chamber | For strain isolation, plating and all microbiological assays |
Bioprocessing Laboratory | Ultrasonication equipment | For pretreatment and breakdown of complex organic matter |
Bioprocessing Laboratory | Phase contrast microscope | To assess contamination during fermentation and for general observation of microbial growth on petris |
Bioprocessing Laboratory | Peristaltic pumps | For transfer of different substrates and fluids into the fermenter |
Bioprocessing Laboratory | Nitrogen evaporator | To concentrate and dry precious bioproduct samples under nitrogen |
Downstream Bioprocessing Laboratory | Ultrafiltration equipment | For extraction and concentration of different components of fermentation broths |
Downstream Bioprocessing Laboratory | Ultrasonication | For extraction of intracellular material |
Downstream Bioprocessing Laboratory | Lyophilization | Freeze-drying of liquid samples for pre-formulations, analysis and storage |
Downstream Bioprocessing Laboratory | SDS-PAGE | For data profiling of different protein components in the extracted fermentation broths |
Downstream Bioprocessing Laboratory | Microfiltration | For filtration of different fermented broths for analysis |
Downstream Bioprocessing Laboratory | Nanospray dryer | For nanoformulation of different types, such as encapsulation, powder and emulsion |
Peripheral Analytical Laboratory | UV-Vis spectrophotometer, including microplate reader | For analysis of microbial growth, enzymes and chemicals |
Peripheral Analytical Laboratory | Nanosizer, including autotitration and zeta potential measurements | For analysis of particle size in the nanometer range |
Peripheral Analytical Laboratory | Water bath for incubation experiments during analysis | To facilitate reactions during analysis |
Peripheral Analytical Laboratory | Accelerated extraction system, including high pressure | For extraction of organic compounds from complex matrices, such as waste water, wastes and soils |
Peripheral Analytical Laboratory | Microwave extraction | For extraction of organic compounds in small volumes from solid and liquid media |
Private and public sector research partners
- La Barberie Inc.
- Lallemand Inc.
- Magnus Inc.
- Aliments Breton Inc.
Additional information
Title | URL |
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Satinder Kaur Brar | http://www.ete.inrs.ca/satinder-kaur-brar |
The fabulous destiny of waste | http://www.planete.inrs.ca/biovalorisation |
Revolution in the charcuterie industry: spices such as cumin replace nitrites | http://www.radio-canada.ca/emissions/bien_dans_son_assiette/2012-2013/chronique.asp?idChronique=290… |
Spices to replace nitrites in deli meats, what a great idea! | http://www.ete.inrs.ca/actualites/des-epices-pour-remplacer-les-nitrites-dans-les-charcuteries-quel… |