Flow cytometry, mass cytometry, cell sorting and analytical flow cytometry services and instrumentation
We provide access to state-of-the-art analytical flow cytometers, mass cytometers and high-speed cell sorters and cell separators. Facility staff members operate and maintain the instruments, assist researchers in protocol selection and development, and train students, post-doctoral fellows and technologists how to operate the analytical cytometers and how to use data analysis software. In addition, we provide phospho-flow analysis, flow cytometry acquisition services and data analysis services.
The SickKids-UHN Flow Cytometry Facility also has a location at The Hospital for Sick Children’s Peter Gilgan Centre for Research and Learning (PGCRL).
Analytical flow cytometry (assisted and unassisted), phospho-flow analysis, cell sorting, automated magnetic cell separation, CyTOF mass cytometry (analytical mass cytometry/validated mass cytometry antibodies/antibody metal labeling service), flow cytometry training, flow and mass cytometry data analysis, consultations, protocol development.
- Agriculture, animal science and food
- Fisheries and aquaculture
- Forestry and forest-based industries
- Healthcare and social services
- Life sciences, pharmaceuticals and medical equipment
- Ocean industries
Specialized labs and equipment
| Equipment | Function |
|---|---|
| BD FACSAria II (2) | 11-17 colour cell sorters |
| BD FACSAria III (2) | 17-colour cell sorter |
| Becton Dickinson Influx | Eight-colour cell sorter |
| Becton Dickinson LSR II (2) | 14-18 colour analytical flow cytometer |
| BD FACSCanto II (2) | Eight-colour analytical flow cytometer |
| Miltenyi Biotec AutoMACS Pro | Automated magnetic cell separator |