Metabolomics Core Facility

University of Ottawa, Ottawa, Ontario
What the facility does

Provides services in targeted metabolite profiling, untargeted metabolomics and stable isotope tracing analyses

Areas of expertise

Our core facility works closely with our academic and industrial clients to provide mass spectrometry-based solutions to separate, detect and quantify various small molecules. Specialized in water soluble metabolites we provide services from project conceptualization to publication. Our targeted methods have been optimized to provide a broad coverage of many different compound classes, effectively providing a wide look at cellular metabolism while limiting the number of sample injections.

Research services

Project design, grant applications, running samples, processing data, data analysis and help with interpretation. Method development is possible depending on our core’s priorities.

Sectors of application
  • Life sciences, pharmaceuticals and medical equipment
Equipment Function
Agilent 6545B Q-TOF LC/MS Quadrupole Time-of-Flight LC/MS (Liquid Chromatography/Mass Spectrometry). Targeted metabolite profiling, untargeted metabolomics and stable isotope tracing analyses.
Agilent 6470A LC-QQQ MS Triple quadrupole LC/MS (Liquid chromatography/Mass Spectrometry).Targeted metabolite profiling using dedicated ion-pairing chromatography.
Agilent 5977B GC/MSD Gas chromatography/mass spectrometry (GC/MS) detection. Targeted metabolite profiling.
Acid-Resistant Refrigerated CentriVap Vacuum Concentrator Unique refrigerated speedvac for sample prep of thermolabile metabolites
Agilent Mass Profiler Professional (MPP) software Software for mass spec data analysis
Agilent MassHunter Qualitative and Quantitative software Data analysis
Agilent MassHunter VistaFlux Software Qualitative Flux Analysis
Agilent MassHunter Profinder Batch-processing feature extraction software