University of Victoria-Genome BC Proteomics Centre (PC)

University of Victoria, Victoria, British Columbia
What the facility does

Proteomics and metabolomics analytical mass spectrometry

Areas of expertise

The UVic-Genome BC Proteomics Centre (PC) is the longest operating protein and metabolomics core facility in Canada, providing analytical mass spectrometry services to academic, industrial and government laboratories for over 30 years. The PC has performed proteomics and metabolomics services for more than 2,000 research projects in health, agriculture, energy, environment and forestry. The Centre’s technology development program supports service and collaborative research projects in the application and development of novel methods in the areas of biomarker identification and characterization, protein characterization, as well as bioinformatics. The Centre offers significant expertise in supporting custom projects that require novel solutions to answer our user’s research question.

Research services
  • Untargeted quantitative proteomics (e.g. iTRAQ and Label-free, Data Independent Acquisition)
  • Targeted quantitative proteomics providing absolute concentration
  • Untargeted metabolomics detecting >5,000 metabolite features
  • Targeted metabolomics using >30 metabolite class or pathway specific assays, for >1,100 metabolites
  • Protein characterization including hydrogen-deuterium exchange, protein cross-linking, surface modifications, characterization of post-translational modifications
  • Bioinformatics and statistical services to aid in experimental design, data interpretation and visualization
Sectors of application
  • Agriculture, animal science and food
  • Chemical industries
  • Energy
  • Environmental technologies and related services
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Agilent Triple Quadrupole Mass Spectrometers (6490/6495B) (4)

Targeted (MRM) proteomics

Thermo Scientific Orbitrap Mass Spectrometer (Velos pro and Fusion)

  • Untargeted proteomics
  • Untargeted metabolomics
  • Structural proteomics
  • Functional proteomics

Bruker Ultraflex III MALDI TOF/TOF Mass Spectrometer

  • MALDI based proteomic characterization and quantitation
  • MALDI imaging

Tecan Liquid Handling System

Automated sample processing

Agilent Bravo Liquid Handling Platform

Automated sample processing

Biotage SYRO II Peptide Synthesizer

High-throughput peptide synthesis

Agilent Single Quadrupole and Liquid Chromatography Mass Spectrometer (LC/MS system)

Peptide purification