Manitoba Centre for Proteomics and Systems Biology

University of Manitoba, Winnipeg, Manitoba
What the facility does

Analysis of biological materials using mass spectrometry; functional genomics to define protein roles in biology; monoclonal antibody production

Areas of expertise

The Manitoba Centre for Proteomics and Systems Biology harbors world-class expertise in mass spectrometry (MS), proteomics, chromatography, data analysis, and translational research. The lab has state-of-the-art mass spectrometry equipment and data analysis infrastructure to provide a range of services for researchers from academia, government, and industry. The team works with a wide spectrum of biological materials from human, mouse, other mammals, microbes, and plants. Applications include proteome-wide quantitation of proteins and post-translational modifications to identify regulated proteins and pathways, studying protein-protein interactions, biomarker discovery and validation. The team employs various strategies for quantitative mass spectrometry, such as label free quantitation (data-dependent acquisition and data-independent acquisition), chemical labeling for multiplexing (tandem mass tag, TMT or stable isotope labeling by amino acids in cell culture, SILAC), and targeted MS (parallel-reaction monitoring, PRM). The team has strong expertise in data analysis of omics and multi-omics datasets including the use of machine learning and artificial intelligence. The team can provide support with experimental design, bioinformatics analysis of complex datasets, and meeting publishing guidelines for proteomics datasets.

Interactions are on a cost-recovery basis and can be either strictly service or on a collaborative basis.

Research services
  • Complete solutions from assisting with study design to identification of statistically significantly regulated proteins, post-translational modifications, and protein interaction partners, including the identification of potential marker panels.
  • Quantitation and identification of proteins and post-translational modifications.
  • Identification of protein-protein interaction partners.
  • Characterization of purified proteins (purity, sequence, mapping of post-translational modifications).
  • Assay development for protein panels.
  • Machine learning assisted analysis of complex omics datasets (proteomics, multi-omics, single cell transcriptomics). 
  • Olink-based measurement of protein panels. 
Sectors of application
  • Agriculture, animal science and food
  • Arts and cultural industries
  • Chemical industries
  • Defence and security industries
  • Education
  • Energy
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Healthcare and social services
  • Information and communication technologies and media
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Bruker timsTOF Pro2 Mass Spectrometer

Couples trapped ion mobility spectrometry (TIMS) to high-resolution time-of-flight (TOF) mass spectrometry. Quantifies proteins and post-translational modifications using data-independent acquisition (DIA). Processes 30-100 samples per day.

Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer with FAIMS interfaceField Asymmetric Waveform Ion Mobility Spectrometry (FAIMS). Quantifies proteins and post-translational modifications. Maximizes sensitivity with parallel reaction monitoring (PRM). 
Thermo Scientific KingFisher Flex Purification System

Automated sample preparation for high-throughput applications.

Olink Signature Q100 system

Benchtop system for protein biomarker analysis. Antibody-based high throughput targeted proteomics.
  • National Institutes of Health
  • University of Miami
  • University of Alberta
  • University of Adelaide
  • The University of British Columbia