Manitoba Centre for Proteomics and Systems Biology

University of Manitoba, Winnipeg, Manitoba
What the facility does

Analysis of biological materials using mass spectrometry; functional genomics to define protein roles in biology; monoclonal antibody production

Areas of expertise

The Manitoba Centre for Proteomics and Systems Biology provides a range of capabilities for researchers from academic, government and commercial settings. We offer expertise in sample preparation and analysis for a spectrum of biological materials. These include, but are not limited to, biomarker discovery and validation in human, murine and other mammalian systems as well as numerous microbes and plants. We also produce custom monoclonal antibodies and provide assistance in assay development for immunoassays or quantitative mass-spectrometry-based approaches (e.g. reaction monitoring).

A major area of research involves the isolation and identification of protein post-translational modifications. Associated with this is the development of software for the prediction of chromatographic properties for inclusion in analytical software.

Interactions are on a cost-recovery basis and can be either strictly service or on a collaborative basis.

Research services

Study design for proteome analysis, protein separation for analysis, post-translational modification analysis, mass-spectrometry-based analysis of proteome, assay development and quantitative protein measurements using mass spectrometry, monoclonal antibody production

Sectors of application
  • Agriculture, animal science and food
  • Chemical industries
  • Defence and security industries
  • Education
  • Energy (renewable and fossil)
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Healthcare and social services
  • Information and communication technologies and media
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Equipment

Function

AB Sciex TripleTOF 5600 Mass Spectrometer

Comprehensive analysis of all peptides in sample with MS/MS for post-translational modifications and development of quantitative approaches.

ORFeome

Human orfeome releases 1 and 2.

TRC and GIP Z lentiviral libraries

Human and mouse shRNAmir libraries.

Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer

  
  • National Institutes of Health
  • University of Miami
  • University of Alberta
  • University of Adelaide
  • The University of British Columbia