The Centre for the Analysis of Genome Evolution and Function (CAGEF)

University of Toronto, Toronto, Ontario
What the facility does

Promotes and facilitates interdisciplinary research in comparative, evolutionary, and functional analyses of genomes and proteomes

Areas of expertise

The Centre for the Analysis of Genome Evolution and Function (CAGEF) offers comprehensive experimental design, research, and analysis services for the analysis of genomes, transcriptomes, microbiomes, and proteomes. Our state-of-the-art facility and experienced technical and bioinformatics staff provide a broad range of genomics, proteomics, and bioinformatic services, including both standard analyses, and techniques developed by our team. We have particular expertise in microbial, plant, and environmental systems.

Research services
  • Whole genome sequencing
  • Genome analysis
  • Single nucleotide polymorphism detection
  • Transcriptome analysis
  • Small RNA analysis
  • Chromatin immunoprecipitation assays
  • Microbiome sequencing
  • Metagenomics
  • Metatranscriptomics
  • Protein molecular weight determination
  • Protein identification
  • Protein post-translational modification determination
  • Protein pull-down assays
  • Protein tandem affinity purification tagging studies
  • Detailed proteome profiling of organisms
  • Bioinformatic services
Sectors of application
  • Agriculture, animal science and food
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Life sciences, pharmaceuticals and medical equipment
  • Mining, minerals and metals
  • Ocean industries
EquipementFunction
Illumina NextSeq 500 v2 Desktop SequencerWhole genome sequencing, RNA sequencing, exome sequencing,  metagenomics, metatranscriptomics
Illumina MiSeq v2 Personal SequencerMicrobiome sequencing, high throughput amplicon sequencing, small RNA sequencing, chromatin immunoprecipitation assays
LTQ XL OrbitrapTM Hybrid Ion Trap Mass SpectrometerProtein and post-translational modification identification
Thermo Scientific EASY-nano LC™ 1200 SystemProtein and post-translational modification identification
Oxford Nanopore MinION & PromethIONLong-read genome sequencing
  • Agriculture & Agri-food Canada
  • Public Health Ontario
  • Vineland Research & Innovation Centre