Princess Margaret Genomics Centre (PMGC)

University Health Network, Toronto, Ontario
What the facility does

Genomics, epigenomics and functional genomics services and technology development. Specializing in clinically relevant samples and single cell genomics analysis, and spatial transcriptomics analysis.

Areas of expertise

The Princess Margaret Genomics Centre (PMGC) has one of the broadest technology profiles in Canada. We are dedicated to providing high quality genomics services to researchers in Canada and around the world. Our approach is to provide the right technology to explore the problem ensuring the best possible data. We are experienced technology innovators as well as service providers.  

We are the leading provider of single cell genomics technologies in the country with particular emphasis on transcriptomics, epigenomics, and coding mutations. We specialize in working with a wide range of sample types including those from formalin-fixed paraffin-embedded samples, fine needle aspirates, frozen tissue samples, viably cryopreserved samples, and flow sorted cells.

We provide a consultative approach to help identify the best strategy to ensure our customers obtain the best possible data for their research programs.

Research services
  • Project consultation
  • Deep-sequencing/next-generation sequencing (NGS) applications and services
  • Exome-sequencing
  • RNA-sequencing
  • Single cell mRNA-seq (multiple platforms)
  • Single cell immune profiling (TCR/BCR)
  • Single cell DNA-coding mutations
  • Single cell protein profiling (CiteSeq/TotalSeq)
  • Single cell ATAC-seq
  • Single cell Multiome (transcriptome and ATAC from same cell)
  • Spatial transcriptomics
  • ChIP-sequencing
  • ATAC-Seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing)
  • Targeted resequencing
  • CRISPR library generation and sequencing
  • Low-C sequencing
  • Methylation sequencing and microarrays
  • NanoString services
  • Illumina microarrays
  • Fluidigm single cell qPCR (quantitative polymerase chain reaction)
  • Luminex® assays
  • Digital droplet PCR (polymerase chain reaction)
  • Nucleic acid sample quality assessment
Sectors of application
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
Specialized labEquipmentFunction
Deep Sequencing LabIllumina NovaSeq 6000 SystemWhole-genome sequencing. Deep sequencing for genomics applications including Exome, RNA, ChIP, ATAC, and Methyl Sequencing.
 Illumina HiSeq 2500 SystemHigh-throughput sequencing system
 Illumina NextSeq 500 SystemWhole-genome sequencing
 Illumina MiSeq SystemBenchtop sequencer
 Ion Torrent ProtonPGM – Personal Genome Machine
Single Cell Lab10X Genomics Chromium instrument, Fluidigm C1 instrumentDedicated facilities for capture of cells via droplet or microfluidic capture, amplification of RNA and DNA from minute samples and processing of single cell RNA-seq and ATAC-seq applications
Targeted GenomicsNanoString nCounter platformPrecise and reproducible quantification of either RNA or DNA
 Fluidigm Biomark HD SystemAutomated, high-performance PCR/qPCR system
 Bio-Rad QX200™ Droplet Digital PCR SystemPolymerase Chain Reaction (PCR)
 Illumina MiSeq SystemBenchtop sequencer