Bacterial strain characterization (i.e. molecular typing) and bacterial identification
Major goals of the Listeriosis Reference Service (LRS) include investigation of listeriosis cases and maintenance of a national collection of isolates. The LRS houses a comprehensive molecular epidemiological database of all isolates in Canada for use as a resource for outbreak investigations, research and other microbiological investigations. The pulsed-field gel electrophoresis (PFGE), ribotype and serological profiles (genotypic and phenotypic molecular fingerprints) are being established and stored for clinical, food, environmental, and possibly animal strains of L. monocytogenes. The LRS pursues research activities for investigation and implementation of other molecular methods for characterizing L. monocytogenes isolates. Other molecular-based methods, such as multiple loci variable number of tandem repeats analysis (MLVA) and DNA sequence-based typing schemes are being investigated on selected diversity sets.
The LRS participates in PulseNet Canada for real-time molecular surveillance for listeriosis, and utilizes PFGE typing for outbreak investigations. The molecular epidemiological data, timely coordination and exchange of information is used to help reduce the incidence of listeriosis in Canada.
- Provide access to the RiboPrinter
- Assist physicians and Provincial Departments of Health when foodborne listeriosis is suspected
- Examine suspect foods and clinical specimens submitted for analysis
- Rapidly alert responsible agencies when commercial foods are involved
- Maintain reference cultures of Listeriae
- Liaise with centres that have similar interests and responsibilities in anada and abroad
- Agriculture, animal science and food
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
|Ribotyping is a molecular technique that takes advantage of unique DNA sequences to differentiate strains of bacteria